All 42 research tools

Every analysis tool is free and runs entirely in your browser — your data never leaves your device. Pick a tool to open it directly.

• Cell Counter & Image SegmentationFree online cell counter. Upload a micrograph and count cells automatically with area, circularity and confluence. Images never leave your device.
• Western Blot Densitometry AnalyzerLane densitometry with rolling-ball background subtraction and loading-control normalisation against GAPDH, β-actin or tubulin.
• qPCR ΔΔCt Calculator — Biological vs Technical ReplicatesFree qPCR fold change calculator that handles replicates correctly: technical replicates are averaged within each biological replicate, and n comes from biological replicates only. Imports QuantStudio and Bio-Rad CFX exports. Livak 2^-ddCt and Pfaffl efficiency correction, MIQE-aware.
• qPCR Efficiency Calculator (Standard Curve)Free qPCR amplification efficiency calculator. Enter a serial dilution standard curve and get E = 10^(-1/slope) - 1, percent efficiency, R2 and MIQE pass/fail checks, plus the ΔCt-slope test that decides whether 2^-ΔΔCt is valid.
• Nucleic Acid Concentration Calculator (A260)Free DNA/RNA concentration calculator from A260 with Beer-Lambert factors (dsDNA 50, ssDNA 33, RNA 40 ug/mL), A260/A280 and A260/A230 purity interpretation, dilution factor and ng/uL to nM molarity conversion.
• 4PL IC50 & Dose-Response CalculatorFree IC50 and EC50 calculator. Four-parameter logistic (4PL) curve fitting with Hill slope, confidence intervals and ROUT outlier removal.
• ELISA & BCA Standard Curve CalculatorLinear regression standard curves for BCA, Bradford, ELISA and qPCR with R², blank subtraction and batch unknown interpolation.
• Drug Synergy Calculator — Bliss Independence ScoreFree drug synergy calculator. Scores Bliss independence across a multi-dose combination matrix and flags synergy or antagonism well by well. Runs in your browser.
• Cell Culture & Doubling Time TrackerPopulation doubling time, confluence tracking and passaging schedule forecasting for adherent and suspension culture.
• Flow Cytometry Panel DesignerMulti-laser excitation and emission spectral overlap validation with spillover compensation warnings.
• PCR Primer DesignerPrimer design with SantaLucia 1998 nearest-neighbour Tm, GC clamp checking, hairpin and dimer screening.
• Sanger Trace Chromatogram ViewerFour-colour AB1 chromatogram rendering with Phred quality scores and reference variant calling.
• CRISPR gRNA DesignerFree CRISPR guide RNA design tool. Finds every PAM site for SpCas9, SaCas9 and Cas12a, ranks candidates and outputs cloning oligos.
• CRISPR Indel Analysis & Frameshift CallerAligns an edited clone against its reference to call indel size, position and reading-frame consequence.
• Multiple Sequence Alignment (MSA) ViewerProgressive Needleman-Wunsch alignment with affine gap penalties, Clustal consensus notation and FASTA export.
• Plasmid Map Viewer & Restriction SitesInteractive circular vector maps with feature arcs and single-cutter restriction enzyme site annotation.
• Gibson & HiFi Assembly PlannerHomology arm designer for Gibson and NEBuilder HiFi assembly with SantaLucia nearest-neighbour Tm matching.
• 3D Macromolecular PDB ViewerWebGL protein structure visualisation with secondary structure ribbons, AlphaFold pLDDT colouring and residue inspection.
• DNA Reverse Complement, Translate & ORF FinderReverse complement, translation, six-frame ORF finding, GC content, restriction digest with Dam/Dcm methylation screening, and codon optimisation.
• siRNA Knockdown DesignersiRNA candidate design following Tuschl rules with asymmetric 3′ overhangs, GC filtering and seed-region screening.
• Protein MW, pI & Extinction CalculatorMolecular weight, isoelectric point by Henderson-Hasselbalch iteration, GRAVY index and Pace 1995 A280 extinction coefficient.
• Oligo & Primer Resuspension CalculatorMolecular weight, ε260 extinction and exact nuclease-free water volumes for resuspending lyophilised primers.
• Dilution & Molarity Calculator (C₁V₁ = C₂V₂)C₁V₁ = C₂V₂ dilution calculator: stock in mM, target in µM, final volume in mL, giving the stock and diluent volumes to pipette.
• Buffer & Solution Recipe GeneratorStep-by-step recipes and pH adjustment for PBS, Tris-HCl, HEPES, SDS-PAGE, TAE and agarose gels.
• PCR Master Mix CalculatorReaction master mix volumes with pipetting excess, per-component breakdown and plate scaling.
• Lentiviral MOI & Titre CalculatorPoisson transduction probability and viral stock volumes for a target multiplicity of infection.
• Transfection Calculator — DNA & Reagent VolumesFree transfection calculator. Lipofectamine 3000 amounts from the manufacturer table for 96-well to T-175, and area-based scaling of your own optimised condition for any reagent. Runs in your browser.
• Cell Line & Mycoplasma Testing RegistryTrack your lab’s cell lines with passage number, source and mycoplasma test dates. Status ages automatically: clear, due at 30 days, overdue at 60.
• Repeated Measures ANOVA & Mixed Model CalculatorRepeated-measures ANOVA with Greenhouse-Geisser correction and linear mixed models for unbalanced designs with missing values, alongside t-tests, one-way ANOVA, Tukey, Kruskal-Wallis and Dunn. Exact p-values, free, runs in your browser.
• Sample Size & Statistical Power CalculatorFree sample size and power calculator. Work out the n you need from a Cohen’s d effect size, or the power a planned experiment actually has, with grant-ready justification text to paste into a proposal. Runs in your browser.
• Kaplan-Meier Survival Curve GeneratorProduct-limit survival estimation with right-censoring, hazard ratios and the Mantel-Cox log-rank test.
• RNA-seq Volcano Plot VisualizerInteractive −log₁₀(p-adj) versus log₂ fold-change volcano plots from your own DESeq2, edgeR or limma results table. Thresholding and plotting only — no differential-expression model is run.
• Electronic Lab Notebook (ELN)Hash-chained electronic lab notebook with timestamped signatures and exportable audit certificates. 21 CFR Part 11-ready.
• -80C Freezer Inventory & Cryo Box LocatorSearchable -80 °C sample inventory: box, row and column positions, type, concentration and volume, with CSV export.
• Antibody Validation TrackerAntibody inventory with validated application matrix (WB, IHC, IF, Flow), RRID lookup, lot numbers and dilution logs.
• Reagent Ordering & Inventory TrackingFree reagent ordering and inventory tracker. Purchase orders, vendor catalogue numbers, unit pricing and grant budget allocation in one place. Runs in your browser, no account needed.
• Publication Figure Panel Composer (300 DPI)Journal-ready multi-panel figure layout with scale bars, panel lettering and high-resolution export.
• Methods Section WriterDrafts a Materials and Methods paragraph from your experiment details, leaves unsupplied details as placeholders, and lists what ARRIVE, MIQE and SAMPL would still ask for.
• Protocol & SOP WriterA library of twelve step-by-step reference protocols, from CRISPR RNP electroporation to ChIP-qPCR, with an optional AI assistant for bench questions.
• Pre-Submission Manuscript AuditChecks a manuscript for statistical power, blinding, reagent authentication and data availability before submission.
• Lab Team & Collaboration HubShared protocol library, peer-review requests and team invites for your lab group.
• Share & Export Research DataShare SciKeep tools with collaborators and your wider lab group.