SciKeep — research tools for life scientists that tell you when your analysis is wrong
Most tools compute whatever you ask and stay quiet. SciKeep catches the errors that get papers retracted — pseudoreplication, unstable reference genes, p-values your design cannot support — and refuses to print a number it cannot stand behind. Everything runs in your browser; images and raw data never leave your device.
All 42 tools
Cell Counter & Image Segmentation
Free online cell counter. Upload a micrograph and count cells automatically with area, circularity and confluence. Images never leave your device.
Western Blot Densitometry Analyzer
Lane densitometry with rolling-ball background subtraction and loading-control normalisation against GAPDH, β-actin or tubulin.
qPCR ΔΔCt Calculator — Biological vs Technical Replicates
Free qPCR fold change calculator that handles replicates correctly: technical replicates are averaged within each biological replicate, and n comes from biological replicates only. Imports QuantStudio and Bio-Rad CFX exports. Livak 2^-ddCt and Pfaffl efficiency correction, MIQE-aware.
qPCR Efficiency Calculator (Standard Curve)
Free qPCR amplification efficiency calculator. Enter a serial dilution standard curve and get E = 10^(-1/slope) - 1, percent efficiency, R2 and MIQE pass/fail checks, plus the ΔCt-slope test that decides whether 2^-ΔΔCt is valid.
Nucleic Acid Concentration Calculator (A260)
Free DNA/RNA concentration calculator from A260 with Beer-Lambert factors (dsDNA 50, ssDNA 33, RNA 40 ug/mL), A260/A280 and A260/A230 purity interpretation, dilution factor and ng/uL to nM molarity conversion.
4PL IC50 & Dose-Response Calculator
Free IC50 and EC50 calculator. Four-parameter logistic (4PL) curve fitting with Hill slope, confidence intervals and ROUT outlier removal.
ELISA & BCA Standard Curve Calculator
Linear regression standard curves for BCA, Bradford, ELISA and qPCR with R², blank subtraction and batch unknown interpolation.
Drug Synergy Calculator — Bliss Independence Score
Free drug synergy calculator. Scores Bliss independence across a multi-dose combination matrix and flags synergy or antagonism well by well. Runs in your browser.
Cell Culture & Doubling Time Tracker
Population doubling time, confluence tracking and passaging schedule forecasting for adherent and suspension culture.
Flow Cytometry Panel Designer
Multi-laser excitation and emission spectral overlap validation with spillover compensation warnings.
PCR Primer Designer
Primer design with SantaLucia 1998 nearest-neighbour Tm, GC clamp checking, hairpin and dimer screening.
Sanger Trace Chromatogram Viewer
Four-colour AB1 chromatogram rendering with Phred quality scores and reference variant calling.
CRISPR gRNA Designer
Free CRISPR guide RNA design tool. Finds every PAM site for SpCas9, SaCas9 and Cas12a, ranks candidates and outputs cloning oligos.
CRISPR Indel Analysis & Frameshift Caller
Aligns an edited clone against its reference to call indel size, position and reading-frame consequence.
Multiple Sequence Alignment (MSA) Viewer
Progressive Needleman-Wunsch alignment with affine gap penalties, Clustal consensus notation and FASTA export.
Plasmid Map Viewer & Restriction Sites
Interactive circular vector maps with feature arcs and single-cutter restriction enzyme site annotation.
Gibson & HiFi Assembly Planner
Homology arm designer for Gibson and NEBuilder HiFi assembly with SantaLucia nearest-neighbour Tm matching.
3D Macromolecular PDB Viewer
WebGL protein structure visualisation with secondary structure ribbons, AlphaFold pLDDT colouring and residue inspection.
DNA Reverse Complement, Translate & ORF Finder
Reverse complement, translation, six-frame ORF finding, GC content, restriction digest with Dam/Dcm methylation screening, and codon optimisation.
siRNA Knockdown Designer
siRNA candidate design following Tuschl rules with asymmetric 3′ overhangs, GC filtering and seed-region screening.
Protein MW, pI & Extinction Calculator
Molecular weight, isoelectric point by Henderson-Hasselbalch iteration, GRAVY index and Pace 1995 A280 extinction coefficient.
Oligo & Primer Resuspension Calculator
Molecular weight, ε260 extinction and exact nuclease-free water volumes for resuspending lyophilised primers.
Dilution & Molarity Calculator (C₁V₁ = C₂V₂)
C₁V₁ = C₂V₂ dilution calculator: stock in mM, target in µM, final volume in mL, giving the stock and diluent volumes to pipette.
Buffer & Solution Recipe Generator
Step-by-step recipes and pH adjustment for PBS, Tris-HCl, HEPES, SDS-PAGE, TAE and agarose gels.
PCR Master Mix Calculator
Reaction master mix volumes with pipetting excess, per-component breakdown and plate scaling.
Lentiviral MOI & Titre Calculator
Poisson transduction probability and viral stock volumes for a target multiplicity of infection.
Transfection Calculator — DNA & Reagent Volumes
Free transfection calculator. Lipofectamine 3000 amounts from the manufacturer table for 96-well to T-175, and area-based scaling of your own optimised condition for any reagent. Runs in your browser.
Cell Line & Mycoplasma Testing Registry
Track your lab’s cell lines with passage number, source and mycoplasma test dates. Status ages automatically: clear, due at 30 days, overdue at 60.
Repeated Measures ANOVA & Mixed Model Calculator
Repeated-measures ANOVA with Greenhouse-Geisser correction and linear mixed models for unbalanced designs with missing values, alongside t-tests, one-way ANOVA, Tukey, Kruskal-Wallis and Dunn. Exact p-values, free, runs in your browser.
Sample Size & Statistical Power Calculator
Free sample size and power calculator. Work out the n you need from a Cohen’s d effect size, or the power a planned experiment actually has, with grant-ready justification text to paste into a proposal. Runs in your browser.
Kaplan-Meier Survival Curve Generator
Product-limit survival estimation with right-censoring, hazard ratios and the Mantel-Cox log-rank test.
RNA-seq Volcano Plot Visualizer
Interactive −log₁₀(p-adj) versus log₂ fold-change volcano plots from your own DESeq2, edgeR or limma results table. Thresholding and plotting only — no differential-expression model is run.
Electronic Lab Notebook (ELN)
Hash-chained electronic lab notebook with timestamped signatures and exportable audit certificates. 21 CFR Part 11-ready.
-80C Freezer Inventory & Cryo Box Locator
Searchable -80 °C sample inventory: box, row and column positions, type, concentration and volume, with CSV export.
Antibody Validation Tracker
Antibody inventory with validated application matrix (WB, IHC, IF, Flow), RRID lookup, lot numbers and dilution logs.
Reagent Ordering & Inventory Tracking
Free reagent ordering and inventory tracker. Purchase orders, vendor catalogue numbers, unit pricing and grant budget allocation in one place. Runs in your browser, no account needed.
Publication Figure Panel Composer (300 DPI)
Journal-ready multi-panel figure layout with scale bars, panel lettering and high-resolution export.
Methods Section Writer
Drafts a Materials and Methods paragraph from your experiment details, leaves unsupplied details as placeholders, and lists what ARRIVE, MIQE and SAMPL would still ask for.
Protocol & SOP Writer
A library of twelve step-by-step reference protocols, from CRISPR RNP electroporation to ChIP-qPCR, with an optional AI assistant for bench questions.
Pre-Submission Manuscript Audit
Checks a manuscript for statistical power, blinding, reagent authentication and data availability before submission.
Lab Team & Collaboration Hub
Shared protocol library, peer-review requests and team invites for your lab group.
Share & Export Research Data
Share SciKeep tools with collaborators and your wider lab group.